Retrieval of collected cell material

Retrieval of collected cell material

1) Push the screw needle out of the tip of the cannula. Place the screw against a glass slide (Fig. I) and prepare smears from the material, which are then fixed and stained.

2) Material rolled up in the grooves of the screw is retrieved by rotating the screw needle anti-clockwise against the edge of a sterile glass slide (Fig. J). The fragments are then fixed in formaline. Cell and tissue material thus obtained may be imbedded and treated as an ordinary histological specimen.

3)After removal of the screw needle from the cannula an ordinary syringe should be attached to the cannula and its contents blown out. This material may then be smeared on a glass slide for staining and microscopy or transferred to a bacteriological culture medium.